Effect ph enzymes coursework

Introduction to Enzymes

We think you have liked this presentation. If you wish to download it, please recommend it to your friends in any social system. Share buttons are a little bit lower. Thank you! Published by Peter Malcolm Owens Modified over 3 years ago. Introduction 2. Planning and Preparation 3.


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When finished, assemble in a lab group of 2 students and begin planning your experiment. Unit 1 Reaction Rates. Scientific enquiry D1.

Enzyme Catalase

C1: Using and organising techniques,. A2 Biology Coursework. You will present a written report, of between and words, of an experimental investigation you have devised and carried.


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Investigate how the concentration of a hydrogen peroxide solution affects the speed at which it decomposes to produce oxygen gas. Unit Reaction Rates 1. A student was asked to write a plan of the procedure for an investigation. The entry made in her.

The effect of ph on catalase coursework - Hiweb

Similar presentations. Factors that disrupt protein structure include temperature and pH; factors that affect catalysts in general include reactant or substrate concentration and catalyst or enzyme concentration. The activity of an enzyme can be measured by monitoring either the rate at which a substrate disappears or the rate at which a product forms. At this point, so much substrate is present that essentially all of the enzyme active sites have substrate bound to them. In other words, the enzyme molecules are saturated with substrate.

The excess substrate molecules cannot react until the substrate already bound to the enzymes has reacted and been released or been released without reacting.


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  • Ten taxis enzyme molecules are waiting at a taxi stand to take people substrate on a minute trip to a concert hall, one passenger at a time. If only 5 people are present at the stand, the rate of their arrival at the concert hall is 5 people in 10 minutes.

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    If the number of people at the stand is increased to 10, the rate increases to 10 arrivals in 10 minutes. With 20 people at the stand, the rate would still be 10 arrivals in 10 minutes. The rate would simply be higher 20 or 30 people in 10 minutes before it leveled off.

    Enzymes II - Temperature and pH

    This is true for any catalyst; the reaction rate increases as the concentration of the catalyst is increased. To some extent, this rule holds for all enzymatic reactions.

    The Effect of pH on Enzyme Activity

    This fact has several practical applications. We sterilize objects by placing them in boiling water, which denatures the enzymes of any bacteria that may be in or on them. We preserve our food by refrigerating or freezing it, which slows enzyme activity. Because most enzymes are proteins, they are sensitive to changes in the hydrogen ion concentration or pH. Ionizable side groups located in the active site must have a certain charge for the enzyme to bind its substrate.

    An enzyme exhibits maximum activity over the narrow pH range in which a molecule exists in its properly charged form. With the notable exception of gastric juice the fluids secreted in the stomach , most body fluids have pH values between 6 and 8. Not surprisingly, most enzymes exhibit optimal activity in this pH range.

    However, a few enzymes have optimum pH values outside this range. For example, the optimum pH for pepsin, an enzyme that is active in the stomach, is 2. Initially, an increase in substrate concentration leads to an increase in the rate of an enzyme-catalyzed reaction. As the enzyme molecules become saturated with substrate, this increase in reaction rate levels off.